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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
101Bio//(100 rxn)/W2569-100
产品编号:W2569-100
市  场 价:¥3980.00
场      地:美国(厂家直采)
产品分类: 分子类>其他分子产品>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$199.00
品      牌: 101Bio
公      司:101Bio
公司分类:
101Bio//(100 rxn)/W2569-100
商品介绍
HiFiScript 1st Strand cDNA Synthesis Kit

Name

HiFiScript 1st Strand cDNA Synthesis Kit

Cat. #

W2569-10
$ 39.00
(10 rxn)
W2569-30
$ 79.00
( 30 rxn)
W2569-100
$199.00
(100 rxn)
How to pay with

Application

  • Reverse transcription, 1st strand cDNA synthesis
  • cDNA library construction
This product is for research use only.

Specifications

Optimal Reaction Temperature:42℃
Final cDNA size:Up to 12 kb
Reaction time:30 ~ 50 min.
Reverse Transcriptase:HiFiScript
RNase H Activity:Reduced
Sensitivity:High
cDNA yield:High

Description and features

HiFiScript 1st Strand cDNA Synthesis Kit has been designed for the first-strand cDNA synthesis kit in a two-step RT-PCR experiments. HiFiScript reverse transcriptase in the kit is a new recombinant Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with novel mutations, which endorse HiFiScript the following features:

  • High transcriptional efficiency – higher yields of cDNA
  • High sensitivity – pg level total RNA and/or mRNA can be used to synthesis first strand cDNA.
  • RNase H activity is removed – improved the elongation ability of the enzyme, and increased affinity of transcriptase to RNA template.Generate first strand cDNA up to 12 kb.

The optimized RT Buffer make the reverse transcriptase a wider range of applications, and with high compatibility for subsequent PCR and quantitative PCR experiments.

Shipping / Storage

Ship at 4℃. Store at -20℃ for up to 1 year and avoid freeze-thaw cycles.

Manual (protocol)

101Bio.com HiFiScript 1st Strand cDNA Synthesis Kit

Components

ComponentsAmount
W2569-10W2569-100
HiFiScript, reverse transcriptase, 200 U/μL10 μL100 μL
5X RT Buffer50 μL500 μL
Primer Mix24 μL240 μL
dNTP Mix, 2.5 mM Each50 μL500 μL
DTT, 0.1 M24 μL240 μL
RNase-Free Water1 mL1 mL

RT reaction system

HiFiScript 1st Strand cDNA Synthesis Kit

Note: If the starting RNA is less than 50 ng, RNase Inhibitor (RNasin, Cat.#: W0596) should be added.

Exosome IsolationPurity > 95%
Protein Extraction1 min total protein, 40 min membrane protein
3D Cell Culture Gel30% < mkt="">
PCR Kits50% < mkt="">
Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughput
Endotoxin-Free Plasmid Kitsmaxi, midi and mini-prep
品牌介绍

101bio已经在世界拥有了一系列的生物以及生命科学的产品链,其3D细胞培养领域在世界闻名。其产品优点如下– exosomes方便——无需超速离心– exosomes快捷——不到2个小时即可完成外切体分离纯化– exosomes高回收率——是超速离心的5~10倍– exosomes高纯度——所得外切体纯度高达95%以上– 从2ml的细胞培养上清或200ul血清中即可获得足够的外切体用于下游研究

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