Name | 101Bio High Efficiency Protein Precipitation kit | |||||||||
Cat. # | P5W6 How to pay with  Also can buy from :   | |||||||||
Application | This kit can simply precipitate protein in 25 minutes with high efficiency. This product is for research use only. | |||||||||
Product Size | 30 ml / 30 ml | |||||||||
Description | Protein precipitation is an option for concentrating proteins and removing interfering substances found in protein samples such as salts, lipid and other components that may interfere with downstream applications. One of the most commonly used methods is trichloroacetic acid (TCA) precipitation.This is relatively simple and effective.However, proteins precipitated by TCA method is usually denatured and in many cases the solubility of protein is reduced. Another major disadvantage of traditional TCA/acetone precipitation method is its low efficiency for the samples of low protein concentration.The higher protein concentration sample can be precipitated much more effectively than the lower protein concentration samples.In order to overcome these shortcomings, we have developed this High Efficiency Protein Precipitation Kit, which is a modification of traditional TCA method.It is simple, rapid and highly effective.Low protein concentration samples can be effectively precipitated and concentrated in about 25-30 minutes. The precipitated protein can be re-dissolved readily in detergent containing buffers.
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Shipping / Storage | Ship and store at room temperature. | |||||||||
Shelf Life | 24 months | |||||||||
Manual (protocol) |  | |||||||||
Components |
|
| Name | Cat. # | Size | ||
|---|---|---|---|---|
| 101Bio HRP Substrate Kit | P5W1 | 30 ml /30 ml | ||
| 101Bio Antibody Enhancer | P5W2 | 30 ml | ||
| 101Bio WB Stripping Solution | P5W3 | 250 ml | ||
| 101Bio Ponceau S Stain | P5W4 | 250 ml | ||
| 101Bio Peroxidase Suppressor | P5W5 | 30 ml | ||
| 101Bio High Efficiency Protein Precipitation kit | P5W6 | 30 ml /30 ml | ||
| 101Bio Red Blood Cell Lysis Buffer | P5W7 | 250 ml | ||
| 101Bio WB Blocking Solution | P5W8 | 250 ml | ||
| 101Bio Denaturing Protein Solubilization Reagent | P5W9 | 10 ml | ||
| 101Bio Non-Denatured Protein Solubilization Reagent | P5W10 | 10 ml | ||
| 101Bio Protein Solubilization Reagent for MS | P5W11 | 2 ml | ||
| 101Bio SDS-Remover | P5W12 | 10 ml | ||
| 101Bio Albumin Depletion Reagent for Plasma and Serum | P5W13 | 10 ml | ||
| 101Bio Protein A+G Agarose Beads | W0349 | 1 ml | ||
| 101Bio Protease Inhibitor Cocktail (100x) | W2200 | 1 ml | ||
| 101Bio Yeast Total Protein Extraction Kit for SDS-PAGE | Y203-50 | 50 rxn |
 | Exosome Isolation | Purity > 95% |
| Protein Extraction | 1 min total protein, 40 min membrane protein |
| 3D Cell Culture Gel | 30% < mkt=""> |
| PCR Kits | 50% < mkt=""> |
| Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput |
| Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
101bio已经在世界拥有了一系列的生物以及生命科学的产品链，其3D细胞培养领域在世界闻名。其产品优点如下– exosomes方便——无需超速离心– exosomes快捷——不到2个小时即可完成外切体分离纯化– exosomes高回收率——是超速离心的5~10倍– exosomes高纯度——所得外切体纯度高达95%以上– 从2ml的细胞培养上清或200ul血清中即可获得足够的外切体用于下游研究
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