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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
101Bio//(1 mL, 100 rxn, 20 ul/rxn)/W0690-1
产品编号:W0690-1
市  场 价:¥780.00
场      地:美国(厂家直采)
产品分类: 分子类>其他分子产品>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$39.00
品      牌: 101Bio
公      司:101Bio
公司分类:
101Bio//(1 mL, 100 rxn, 20 ul/rxn)/W0690-1
商品介绍
2x Es Taq Master Mix (with dye)

Name

2x Es Taq Master Mix (with dye)

Cat. #

W0690-1
$ 39.00
(1 mL, 100 rxn, 20 ul/rxn)
W0690-5
$ 99.00
(5 mL, 500 rxn, 20 ul/rxn)
How to pay with

Application

  • Routine PCR
  • T/A cloning
This product is for research use only.

Specifications

Polymerase:Es Taq
Hot Start:N/A
Fidelity:High fidelity
GC-Rich PCR Performance:High
Amplification Range:6 kb
Exonuclease Activity:5´→3´
Reaction Speed:1 kb / 30 seconds
Reaction Format:Master Mix
Product Overhang:3"-A

Description and features

This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer.The concentration is 2x.

Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning.The amplification range of Es Taq is ~ 6 kb.

This unique Master Mix recipe makes the system very reliable.More than 98% of PCR reaction can get successful amplification during the first try.It also works well on complicate templates.

The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.

Shipping / Storage

Ship at 4℃. Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months.

Quality control

This product is tested for no exogenous nuclease activity;no host DNA contamination tested (by PCR);able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months.

Manual (protocol)

101Bio.com 2x Es Taq Master Mix

Components

ComponentsAmount
W0690-1W0690-5
2x Es Taq Master Mix1 mL1 mL x 5
RNase-Free Water1 mL5 mL

PCR reaction system

2x Es Taq Master Mix, convenient, save time and reliable

Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background.

PCR reaction conditions

2x Es Taq Master Mix, convenient, save time and reliable

Note:

  • The recommended annealing temperature is about 5℃ below Tm of primers. If extra bands are observed, higher annealing temperatures should be considered. The absence of product indicates the need for a lower annealing temperature.
  • PCR extension time is depended on the size of target gene sequence.Es Taq DNA polymerase is approximately 1 kb DNA / 30 seconds.
  • The number of PCR cycles will basically depend on the downstream application of the PCR product.

PCR result examination

This Master Mix contains dye for electrophoresis.After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.No need to add loading buffer.

Exosome IsolationPurity > 95%
Protein Extraction1 min total protein, 40 min membrane protein
3D Cell Culture Gel30% < mkt="">
PCR Kits50% < mkt="">
Beta-Hexosaminidase Activity Colorimetric Assay Fast and sensitive, High-throughput
Endotoxin-Free Plasmid Kitsmaxi, midi and mini-prep
品牌介绍

101bio已经在世界拥有了一系列的生物以及生命科学的产品链,其3D细胞培养领域在世界闻名。其产品优点如下– exosomes方便——无需超速离心– exosomes快捷——不到2个小时即可完成外切体分离纯化– exosomes高回收率——是超速离心的5~10倍– exosomes高纯度——所得外切体纯度高达95%以上– 从2ml的细胞培养上清或200ul血清中即可获得足够的外切体用于下游研究

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