Name | 2x GoldStar Best PCR Master Mix (with dye) | ||||||||||||||||||
Cat. # | W0655-1 $ 39.00 (1 mL, 100 rxn, 20 ul/rxn) $159.00 (5 mL, 500 rxn, 20 ul/rxn) How to pay with | ||||||||||||||||||
Application |
This product is for research use only. | ||||||||||||||||||
Specifications |
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Description and features | 2x GoldStar Best PCR Master Mix (with dye) is a convenient premixed 2x concentrated solution for PCR which include GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier. The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity.It also possesses 3´→5´ exonuclease (proofreading) activity. The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase. The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation.Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity. GoldStar Best DNA Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning. The amplification range of Es Taq is ~ 6 kb. This PCR Master Mix contains dye, and can directly run electrophoresis after PCR reaction. | ||||||||||||||||||
Shipping / Storage | Ship at 4℃. Store at -20℃ for up to 1 year and avoid freeze-thaw cycles. Stored at 4℃ for up to 3 months. | ||||||||||||||||||
Quality control | This product is tested for no exogenous nuclease activity;no host DNA contamination tested (by PCR);able to amplify single copy gene from multiple genomes; and no significant enzyme activity decrease after storing at 2 ~ 8oC for 3 months. | ||||||||||||||||||
Manual (protocol) | 101Bio.com 2x GoldStar Best PCR Master Mix (with dye) | ||||||||||||||||||
Components |
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PCR reaction system | Note: The recommended primer concentration for PCR is between 0.1-1.0 µM of each primer. The use of higher concentrations of primers can have higher amplification effect. Low primer concentration will generally ensure cleaner product and lower background. | ||||||||||||||||||
PCR reaction conditions | Note:
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PCR result examination | This PCR Master Mix contains dye for electrophoresis.After PCR, directly load 5 µL of PCR product to agarose gel to run electrophoresis.No need to add loading buffer. |
Exosome Isolation | Purity > 95% | |
Protein Extraction | 1 min total protein, 40 min membrane protein | |
3D Cell Culture Gel | 30% < mkt=""> | |
PCR Kits | 50% < mkt=""> | |
Beta-Hexosaminidase Activity Colorimetric Assay | Fast and sensitive, High-throughput | |
Endotoxin-Free Plasmid Kits | maxi, midi and mini-prep |
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